ATCC15834 Electroporation Competent Cell

ACC-128

ATCC15834 Electroporation Competent Cell

The genotype of ATCC15834 Electroporation Competent Cell is Agrobacterium rhizogenes pRi15834 (agropine type). Agrobacterium rhizogenes is a Gram-negative soil bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. The ATCC15834 Agrobacterium rhizogenes strain is derived from ATCC and contains pRi15834 agrobacterium-type Ri plasmid with a broad host range (gramineae, leguminous, tobacco, etc.).

ATCC15834 Electroporation Competent Cell is suitable for transgenic operations of gramineae, leguminous, tobacco and other plants.

1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.

Store at - 80 °C for 12 months.

ATCC15834 Electroporation: 50 μL/tube * 10 tube/50 tube.

Transformation efficiency of ATCC15834 Electroporation Competent Cell using pCAMBIA2301 plasmid with 50 μg/mL kan is >10^5 cfu/μg DNA. Transformation efficiency is reduced to half when the plate contains 50 μg/mL kan and 20 μg/mL rif.