AAV Vector Building Service

Adeno-associated virus (AAV) is a single-stranded DNA virus belonging to the Parvoviridae family, which can infect a wide range of cells. It is currently the first tool for gene therapy. Based on years of experience in virus packaging platforms, Lifeasible can provide AAV, LV, ADV, and other viruses with different quality standards, which can be widely used to construct various cell line models and in vivo studies in living animals. For AAV virus packaging, we adopt the method of triple plasmid co-transfection and advanced purification process, which can provide high purity, high titer, and multiple serotypes of AAV packaging customization services to meet the individual choice of gene therapy researchers.

Services

Technological route

Delivery

• High concentration of active virus
• Professional lab report
• Sequencing peak graph
• Visualization of vector mapping
• Strict quality control report
• Detailed operation manual

Common vector selection

Advantages

• Reliable quality. High titer, high purity, low endotoxin, high efficiency of in vivo infection.
• Professional service. We provide customized solutions and professional technical guidance for AAV vector construction by overexpression, interference, and CRISPR technology.
• Experienced. Years of experience in virus packaging, constantly expanding stock library.
• Artificial intelligence. Strong AI and bioinformatics team to provide AAV vector development and optimization transformation services.

Quality assurance

Lifeasible is committed to providing high-titer, high-quality AAV, and in addition to routine qPCR testing for AAV titers, we also utilize other quality assurance methods to ensure the quality of AAV. These include viral capsid protein purity testing, viral genome size and purity testing, and viral biological activity testing.

• Viral coat protein purity assay. In addition to the titer test, we also tested the purity of AAV capsid protein by SDS-PAGE. This assay indicates that the purity of AAV capsid protein should have good infectious activity.
• Viral genome size and purity detection. Extract the AAV viral genome and detect the genome size and purity by agarose gel electrophoresis to discriminate the size of the inserted target fragment and the presence or absence of impurities to ensure the quality of AAV. This test indicates that the size of the packaged AAV insert fragment is correct and free of impurities.
• Viral biological activity test. We infected 293T cells with different serotypes of AAV to observe the fluorescence signal. We can also perform an intraviral biological activity assay.

Please feel free to contact us for information.