Description:

The TetX open reading frame [Genbank: JQ990987] was synthesized de novo with codons optimized for Chlamydomonas reinhardtii cytoplasmic expression under the control of constitutive beta 2 tubulin promoter and chlamyopsin1 3' UTR. The BtetX plasmid can be used to select nuclear transformants of Chlamydomonas reinhardtii. It has been proven to work in the cell wall deficient strain MUT-849, however the wild-type cell-walled strain MUT-125 is as sensitive to tetracycline as strain MUT-849. The transformants should be selected in TAP plates containing 15 µg/mL of tetracycline and maintained below a light intensity of 24 µmoles m-2 s-1. Transformed colonies will be visible after 8 days and can resist up to 100 µg/mL of tetracycline.

Bacterial Host Strain:

DH5 alpha

Selectable Marker:

Amp-r