Subategory:

Mutants

Description:

This stock is derivative pf5.6B+. The two pf5 mutants lacked four flagellar polypeptides: radial spoke components 13 and 15, component 1 of the innter dynein arms, and a low molecular weight axonemal protein whose structural localization was unknown. Working with MUT-1331, which was also originally designated pf5, the mutation in that strain was in the FLA14 gene encoding the dynein light chain LC8, and renamed pf5 as fla14-3. MUT-1028 had a wild-type FLA14 gene, capable of rescuing the MUT-1331 phenotype. It is confirmed the expected linkage of the pf5 mutation in MUT-1028 and its derivatives to linkage group III. we conclude that the mutation in MUT-1028 and the one in MUT-1331 are in different genes, but probably closely linked on the same chromosome.

Species:

Chlamydomonas

Locus:

PF5 [DLL1]

Chromosome:

3

Phenotype:

Impaired motility