Subategory:

Mutants

Description:

It was designated 136f. This had been paired with another strain (formerly MUT-1372), and had been identified both as C. reinhardtii. It was concluded that these strains differed sufficiently from the laboratory strain of C. reinhardtii on morphological criteria to warrant description as a new species. Subsequent work has shown that MUT-1373 is fully fertile with C. reinhardtii, but has significant sequence polymorphisms in nuclear, chloroplast and mitochondrial DNA. MUT-1372 on the other hand appears to be an unrelated homothallic strain, probably C. culleus. This strain can grow on nitrate. We recommend MUT-1373 as a polymorphic strain for comparison with C. reinhardtii. Its one drawback is that it sometimes forms "clumpy" cultures as a result of failure of the mother cell walls to lyse after vegetative division. This property may result in poor mating efficiency. In our experience, letting the gamete mixture sit for 24 hours or more usually produces enough release of autolysin to overcome the hatching defect. For an alternative polymorphic strain for nuclear DNA, see MUT-2290, the Minnesota mt- isolate described as less well characterized, but also of possible utility, are MUT-2931 and MUT-2932, a pair of strains isolated in North Carolina.

Species:

Chlamydomonas