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MUT-1375 ac-u-lambda (psbA deletion) mt+
Datasheet
Catalog No: ALS-01773
Product Details
Subategory:
Mutants
Description:
This strain is a mt+ derivative, obtained by successive crosses to MUT-125 wild type of a mutant isolated in a diploid strain. This strain is not arginine-requiring. The mutant shows symmetrical 9 kb deletions in both copies of the psbA gene. This mutant was isolated in an arg2/arg7 diploid strain. Cells were pregrown in 0.5 mM 5-fluorodeoxyuridine in liquid cultures, concentrated, and irradiated with 2 minutes X-ray at 6 krad/min. The irradiated cells were plated on 2 mM arsenate and arsenate-tolerant (non-photosynthesizing) clones were selected.
Species:
Chlamydomonas
Locus:
psbA
Chromosome:
Chloroplast
Phenotype:
Requires acetate
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For Research Use Only. Not For Clinical Use.