Subategory:

Mutants

Description:

The nic-5 mutant requires nicotinamide and cannot use quinolinic acid. mod-1 is a second mutation that blocks utilization of quinolinic acid (in nic-6 mutants) and nicotinic acid (in nic-5 and nic-6). The mod-1 phenotype can be abolished by high ionic strength (50 mM KCl or other salts) but not by osmotically active agenes such as glucose, sucrose, or glycerol. The mod-1 mutation might modify calcium transport, with the change in utilization of nicotinic and quinolinic acids being a secondary effect.

Species:

Chlamydomonas