Subategory:

Mutants

Description:

The ery2a allele was obtained after mutagenesis of a 137c wild-type strain with ethyl methanesulfonate. MUT-895 ery2a mt+ (which is no longer in the collection) was crossed to MUT-124 wild type mt-, and a ery2a mt- product from this cross was crossed to MUT-125 wild type mt+ to give MUT-1711. This strain provides better viability in crosses than the original MUT-895. Since ery2 is easily scored and is a good marker for the sparsely populated linkage group XIV, having this in a strain that crosses well seemed desirable. The ery2 mutants as a group are resistant to 100 micrograms/mL erythromycin on minimal medium, and are cold-sensitive. It was shown that the ERY2 locus corresponds to the gene encoding chloroplast ribosomal protein L22.

Species:

Chlamydomonas

Locus:

ERY2 [RPL22]

Chromosome:

13

Phenotype:

Antibiotic resistant (erythromycin)