Subategory:

Mutants

Description:

This strain should be kept in dim light. Notes: The pg-101 [lor1] mutant was obtained by UV mutation of C. reinhardtii strain 11/32f Levine, mutant Arg2 mt+ and selected for altered color. The phenotypical difference detected at first was the lack in loroxanthin on thinlayer chromatograms of pigment extracts. As separation of lutein and zeaxanthin by HPLC as well as confirmation of the absence of low amounts of alpha-carotene is rather difficult, it took us some years to demonstrate that this mutant is not able to produce alpha-ionone rigns in carotenoids (episilon-rings), which means that the alpha cyclase is inactive or missing. It was renamed to lor-1.

Species:

Chlamydomonas

Phenotype:

Carotenoid deficient; requires acetate