Subategory:

Mutants

Description:

This mutant was isolated by nitrosoguanidine mutagenesis of cells grown in synchronous cultures (12:12 light-dark), at a time approximately 2 hours into the dark cycle. It has a temperature-conditional defect in flagellar function. Cells resemble wild type at permissive temperature (20 °C) but when shifted to 32 °C, the flagella detach rapidly. Cells can initiate flagellar reassembly at 32 °C, but as the flagella elongate they are lost by detachment. Cells of this mutant cannot form colonies at 32 °C under constant light, where rapid growth and cell division are required. Cells grown on an alternating 12 h light-dark cycle divide normally.

Species:

Chlamydomonas

Phenotype:

Impaired motility