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MUT-28 ac17 can1 nic13 pf2 y1 pyr1 msr1 act2 sr1 mt+
Datasheet
Catalog No: ALS-01238
Product Details
Subategory:
Mutants
Description:
MUT-28 and MUT-29 are a very useful pair of mapping strains. Both strains usually give complete tetrads in crosses, and the markers are generally easy to score. The ac17 mutation (see MUT-530) confers a very clean acetate requirement, easily scored by growth on minimal medium (plus nicotinamide in this strain because of the nic13 mutation). The pf2 mutation (see MUT-1025) should be scored as slow-swimming compared to wild type, and nic13 (see MUT-864) should be scored by sensitivity to 3-acetyl pyridine rather than by requirement for nicotinamide.
Species:
Chlamydomonas
Locus:
AC17, ACT2 [RPL36a], CAN1, MSR1, NIC13 [NSN1], PF2, PYR1, SR1
Chromosome:
1,3,4,6,7,9,10,11
Phenotype:
Requires acetate and nicotinamide; resistant to antibiotics and metabolic inhibitors (canavanine; cycloheximide; methionine sulfoximine; pyrithiamine; and streptomycin)
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For Research Use Only. Not For Clinical Use.