Subategory:

Mutants

Description:

It was derived from transformation of strain MUT-1852, a hybrid strain containing the C. smithii chloroplast genome, with plasmid P-344. This plasmid contained a 6.9 kb Eco-Sal C. reinhardtii insert running from psbA intron 4 to the 3' portion of the 23S rRNA gene. Exon 5 of psbA contains the DCMU4 mutation and the rRNA gene contains the er-u-1a mutation. Two diagnostic RFLP fragments in the interval between psbA 5' and rRNA 3' were confirmed to have been derived from C. reinhardtii, rather than the C. smithii chloroplast genome of MUT-1852.

Species:

Chlamydomonas

Phenotype:

Antibiotic and herbicide resistant (DCMU; erythromycin)