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MUT-2968 petA (HCFS) spr sr mt+
Datasheet
Catalog No: ALS-02639
Product Details
Subategory:
Mutants
Description:
This is a site-directed mutation transformed into the signal sequence of the petA gene. MUT-125 was co-transformed with P-183 (carrying sr-u-2-60 spr-u-1-6-2 er-u-37) and a plasmid carrying a site-directed mutation in the signal sequence for the petA gene. Transformants were selected for spectinomycin and streptomycin resistance; the er-u-37 marker was not tested. The HCFS mutation produces a frame shift in the hydrophobic core by inserting a T after nucleotide 29 and removing nucleotide 45. This strain does not grow on minimal medium.
Species:
Chlamydomonas
Locus:
petA
Chromosome:
Chloroplast
Phenotype:
Requires acetate; antibiotic resistant (spectinomycin; streptomycin)
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For Research Use Only. Not For Clinical Use.