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MUT-3022 atpC1 nit1-305 cw15 mt-
Datasheet
Catalog No: ALS-02678
Product Details
Subategory:
Mutants
Description:
This is a null mutation in the ATPC gene encoding the gamma subunit of CF1 ATP synthase. The mutant was obtained by transformation of a nit1-305 cw15 strain with a construct in which a 168 bp Bgl II fragment from the ATPC cDNA was replaced with a plasmid sequence. Transformants were selected for arsenate tolerance and screened for an acetate-requiring phenotype. The mutant was shown to have a defect in the 5' portion of the AtpC gene. It does not accumulate either mRNA or a polypeptide. For site-directed mutations that alter specific amino acids in ATPC, please see MUT-3023 through MUT-3027.
Species:
Chlamydomonas
Locus:
ATPC, NIT1
Chromosome:
6,9
Phenotype:
Requires acetate; wall deficient
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For Research Use Only. Not For Clinical Use.