Subategory:

Mutants

Description:

This strain expresses epitope-tagged alpha1-tubulin to a high and constant level. It was created by transformation. nit1-305 cells were cotransformed with pMN24 (nitrate reductase) and a construct containing the Chlamydomonas alpha1-tubulin gene tagged with a nine amino acid epitope from influenza virus hemagglutinin (HA). To obtain a high level of expression, the alpha1-tubulin promoter was replaced with the Rubisco S2 promoter.

Species:

Chlamydomonas