Subategory:

Mutants

Description:

The gag1 mutation is an allele at the SAG2 locus. Mutants at this locus do not agglutinate with cells of either mating type, but can be mated as mt+ by artificial agglutination with antibodies. Notes: The sterility phenotype is sex-limited (expressed only in mt+ cells) but the gene is unlinked to the mating type locus. The primary defect is in O-glycosylation: at 32 °C, in both mating types. The major O-glycoproteins show increased electrophoretic mobility and altered antigenicity. Cells form clumps on plates and palmelloids in liquid culture. These defects are not observed when cells are grown at 18 °C. However, the sex-limited sterility phenotype is expressed regardless of the growth temperature.

Species:

Chlamydomonas

Phenotype:

Does not mate