This strain contains a site-directed mutation A251G in the psbA gene, and a silent mutation (indicated by * in the strain name) creating an SspI site by substituting a T for a C at position 247. MUT-125 wild-type cells were co-transformed with the psbA plasmid and with plasmid P-228, which confers spectinomycin resistance by a mutation in the gene encoding the chloroplast 16S ribosomal RNA. Strains carrying the A251G mutation are indistinguishable from wild type with respect to herbicide resistance and photoautotrophic growth.
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