This strain was derived as follows: MUT-373, an atpB deletion mutant, was transformed with the wild-type Chlamydomonas atpB gene to produce MUT-3452, which was used as a control for MUT-3453, MUT-3454, and MUT-3455 containing the same atpB gene upstream of three versions of the E. coli recA gene. MUT-3452 was then crossed to MUT-1930 arg2 mt-, and a wild-type progeny clone (i.e. not requiring arginine) was seleted, to yield MUT-3456. MUT-3456 was then transformed with an aadA construct disrupting the chlL gene (Goldschmidt-Clermont), flanked by direct repeats to evaluate recombination frequencies in the chloroplast. MUT-3460 is one of the transformants recovered.
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