Subategory:

Mutants

Description:

The gli mutants were generated using insertional mutagenesis. The background strain was a product from a cross between MUT-1024 pf1 nit1 nit2 mt+ and KK25E5 nit1-305 mt- (which in turn was derived from a cross of a nit1305 mt+ strain to MUT-2453 nit1305 mt-). Non-gliding mutants were selected by their compact colony morphology. The mutants were characterized by ability to adhere to a coverslip, to bind polystyrene beads to the flagellar membrane, and to translocate beads along the length of the flagellum. Class I mutants are capable of all three of these activities. Class II mutants adhere to coverslips but do not bind or translocate beads. Class III mutants bind and translocate beads but do not adhere to coverslips. Class IV mutants are deficient in all three activities. Class V mutants adhere to coverslips, but only by their flagellar tips. They also bind and translocate beads. It was concluded that different glycoproteins are probably involved in coverslip adhesion vs. binding beads shown by classes II and III, and speculated that the Class IV mutants my be deficient in the previously characterized 350 kD protein of the flagellar membrane.The Class I and Class V mutants could have alterations in regulation of gliding or in the gliding motor. This mutant (T1B3) is in Class I.

Species:

Chlamydomonas

Phenotype:

Does not show gliding motility