Subategory:

Mutants

Description:

MUT-3875 through MUT-3893 are a collection of transformants of the T60-3 Rubisco knockout mutant (MUT-4415). MUT-3875 was created by transformation of T60-3 (rbcS-T60-3T60-3) with a modified plasmid pRbcS-2. The original plasmid, a pUC19-vector containing the genomic BamHI/EcoRI fragment encoding rbcS-2 [EMBL/GenBank accession X04471], total length 10.5 kb, was shortened by elimination of an unknown DNA sequence of about 5 kb. Transformation resulted in complementation of T60-3 to normal photoautotrophy. MUT-3875 was used as a control strain for MUT-3876 through MUT-3889, which have mutations in the RBCS transit peptide.

Species:

Chlamydomonas

Locus:

RBCS2