Subategory:

Mutants

Description:

MUT-3875 through MUT-3893 are a collection of transformants of the T60-3 Rubisco knockout mutant (MUT-4415). For MUT-3883, the plasmid used for the transformation leading to MUT-3875 was modified by mutating processing site 2 in the transit peptide of the precursor of the Rubisco small subunit such that Met(46) is exchanged by His(46) and a NdeI restriction site is introduced into the DNA. Transformation resulted in complementation of rbcS-T60-3 to normal photoautotrophy. Synthesis of intermediate sized small subunit is enhanced, but mature small subunit is also formed.

Species:

Chlamydomonas

Locus:

RBCS2