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Description:

MUT-3947 nic7 thi10 MID mt(+)-

Catalog No: ALS-03185

Product Details

Subategory:
Mutants
Description:
An imp1 arg2 strain (MUT-1865) was transformed with pArg7.8 and a 3.5 kb ApaI fragment containing the MID gene. One of these transformants (B28) was crossed to MUT-85 (nic7 mt+) to create a nic7 mt+ strain with a MID transgene. This strain was then crossed to MUT-123 (thi10 mt+ ) to create the strains called K33 and L21 (see MUT-3948). K33 retains the MID transgene, and although genetically plus, it mates as minus.
Species:
Chlamydomonas
Locus:
NIC7, THI10, MID
Chromosome:
6
Phenotype:
Requires nicotinamide and thiamine

For Research Use Only. Not For Clinical Use.