Subategory:

Mutants

Description:

This is the most widely used wall-deficient mutant, and this mutation is present in many strains. It was defined as mutants in which cell walls are absent or produced in greatly reduced quantity compared to wild type. Wall-deficient mutants form flat, liquid colonies on agar, and the cells lyse in a 1% solution of the nonionic detergent NP-40. This is an isolate derived from a cw15 stock. The original stock required 0.2% sodium acetate for growth in liquid and was quite sensitive to pH and/or CO₂; this single clone isolate does not require acetate and is not sensitive.

Species:

Chlamydomonas

Phenotype:

Wall deficient