Subategory:

Mutants

Description:

The osm9 mutant was obtained by insertional mutagenesis of a nit1-305 strain originally derived from MUT-2453, using a NIT1-containing plasmid pGP505, but the NIT phenotype does not cosegregate with the osm phenotype. Please see MUT-4064 for another osm9 isolate that apparently lacks the NIT1 insertion. MUT-4010 was obtained by backcrossing the original mutant three times to wild type MUT-124 or MUT-125 and re-isolating. This strain should be maintained on TAP medium supplemented with 0.06 M sucrose or lactose.

Species:

Chlamydomonas

Phenotype:

Deficient in osmoregulation