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MUT-4048 CR48 (amt4) mt+
Datasheet
Catalog No: ALS-03277
Product Details
Subategory:
Mutants
Description:
This is a spontaneous methylammonium-resistant mutant isolated in the 4A+ background (MUT-4051). The CR48 rmutant has a substitution of a guanine residue for the highly conserved adenine in the right splice junction of intron 2 of the AMT4 transcript. The change shifts the splice site 17 bp to the right, leading to an altered transcript and introducing a frameshift, as verified by sequence analysis of cDNA. The resulting translation product has 85 correct amino acids followed by 32 incorrect amino acids and the same translation stop as in CR02. CR48 has an Amt4 null phenotype.
Species:
Chlamydomonas
Locus:
AMT4
Chromosome:
13
Phenotype:
Inhibitor resistant (methylammonium)
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For Research Use Only. Not For Clinical Use.