Subategory:

Mutants

Description:

The 6F5 mutant was isolated by Steve Myster in the Porter lab using insertional mutagenesis of A54 e18 (MUT-2929). The pMN56 plasmid containing the NIT1 gene integrated into the BOP5 locus encoding the IC138 intermediate chain of the I1 inner arm dynein. The insertion event was associated with the deletion of ~20 kb of genomic gene, including most of the IC138 gene. IC138 fails to assemble into the axoneme along with at least three other I1 dynein subunits (IC97, LC7b, FAP120) known as the IC138 sub-complex. There is also a second uncharacterized motility mutation in the strain.

Species:

Chlamydomonas