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MUT-4368 css1 spr-u-1-6-2 er-u-11 mt+ [Herrin 7151]
Catalog No: ALS-03539
Product Details
Subategory:
Mutants
Description:
The css1 mutation suppresses defects in splicing of the chloroplast 23S (Cr.LSU) intron and also the psbA fourth intron. It was obtained as follows: Mets strain 2137 (MUT-1021, also MUT-3269) was transformed with a construct that contained the spr-u-1-6-2 mutation from plasmid P-183 (a single base pair change in the 16S rRNA) and the chloroplast 23S (Cr.LSU) intron, in which site-directed changes had been made. Transformants were selected by spectinomycin resistance. A transformant defective in Cr.LSU self-splicing (P4125A), which grew slowly and was light-sensitive, was then used to isolate suppressor mutations that restored Cr.LSU splicing.
Species:
Chlamydomonas
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For Research Use Only. Not For Clinical Use.