Subategory:

Mutants

Description:

The css3 mutation suppresses defects in splicing of the chloroplast 23S (Cr.LSU) intron and also the psbA fourth intron. It was obtained as follows: Mets strain 2137 (MUT-1021, also MUT-3269) was transformed with a construct that contained the spr-u-1-6-2 mutation from plasmid P-183 (a single base pair change in the 16S rRNA) and the chloroplast 23S (Cr.LSU) intron, in which site-directed changes had been made. Transformants were selected by spectinomycin resistance. A transformant defective in Cr.LSU self-splicing (P4125A), which grew slowly and was light-sensitive, was then used to isolate suppressor mutations that restored Cr.LSU splicing.

Species:

Chlamydomonas