Subategory:

Mutants

Description:

This mutant was obtained by transformation of strain g1 (nit1, agg1, mt+) with the NIT1 plasmid pMN24. It is phototaxis negative, photoshock positive. It was shown that it is an allele at the BBS7 locus. Notes: The ptx6-1 (T197) and ptx6-2 (T87) strains appear to be identical. The mutation in the BBS7 gene of T87 involves a retrotransposon TOC1 footprint whereas it was reported that the ptx6-1 strain contained pUC119 sequence that segregated with the nonphototactic phenotype but did not encode a functional nitrate reductase gene. It is not known if the bbs7-1 strain (i.e., T87) carries a second, insertional, mutation linked to the BBS4 gene, or if the original ptx6-1 and ptx6-2 alleles were different but ptx6-1 has been lost. In any case, this strain should be referred to simply as bbs7-1. If relevant, anyone working with this strain in the future should check to see if it still contains pUC119 sequence that might be disrupting a second gene.

Species:

Chlamydomonas

Locus:

BBS7

Chromosome:

1