Subategory:

Mutants

Description:

This strain was recovered from a cross between R107-4A mt+ and 76-5EN pf2 mt-. It has been maintained with acetate medium in darkness since its isolation. The R107-4A photosynthesis-competent revertant was selected from the Rubisco rbcL-mRNA deficient 76-5EN nuclear mutant. When R107-4A mt+ was crossed with "wild-type" mt-, only wild-type progeny were obtained. When R107-4A mt+ was crossed with 76-5EN pf2 mt-, the revertant (wild-type) phenotype segregated in a 2:2 pattern with the 76-5EN acetate-requiring phenotype, which indicates that the revertant results from a nuclear-gene mutation. R107-4A likely results from true reversion or intragenic suppression within the 76-5EN gene.

Species:

Chlamydomonas

Locus:

PF2

Chromosome:

11