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MUT-4640 PAADA/76-5EN(11-4) mt-
Catalog No: ALS-03777
Product Details
Subategory:
Mutants
Description:
This is progeny 4 from tetrad 11 from a cross between PAADA mt+ and 76-5EN mt-. The strain can grow on acetate medium with 50 µg/mL spectinomycin in darkness, but dies with 250 µg/mL spectinomycin, indicating that 76-5EN partially blocks expression of aadA from the engineered rbcL promoter. This strain requires acetate for growth because 76-5EN also blocks expression of the native rbcL gene. It has been maintained with acetate medium in darkness since it was isolated.
Species:
Chlamydomonas
Phenotype:
Requires acetate; sensitive to light
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For Research Use Only. Not For Clinical Use.