Subategory:

Mutants

Description:

The 68-11AR nuclear mutant was isolated by screening for temperature-conditional, acetate-requiring mutants. The mutant was crossed with pf2 mt- to confirm mendelian inheritance, and this mt+ progeny clone was retained for further genetic analysis. 68-11AR grows on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The nuclear mutation is not in the Rubisco rbcS genes, but it causes a decrease in Rubisco stability and CO₂/O₂ specificity. Some clones of 68-11AR have been observed to become acetate-requiring at 25 °C after many years of culture. This strain has been maintained with acetate medium in darkness since its isolation.

Species:

Chlamydomonas

Phenotype:

Requires acetate at 35 °C; temperature-conditional