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Description:

MUT-4642 68-11AR mt+ (rbc-2-68-11AR) non-pf2 progeny used for genetic analysis

Catalog No: ALS-03779

Product Details

Subategory:
Mutants
Description:
The 68-11AR nuclear mutant was isolated by screening for temperature-conditional, acetate-requiring mutants. The mutant was crossed with pf2 mt- to confirm mendelian inheritance, and this mt+ progeny clone was retained for further genetic analysis. 68-11AR grows on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The nuclear mutation is not in the Rubisco rbcS genes, but it causes a decrease in Rubisco stability and CO2/O2 specificity. Some clones of 68-11AR have been observed to become acetate-requiring at 25 °C after many years of culture. This strain has been maintained with acetate medium in darkness since its isolation.
Species:
Chlamydomonas
Phenotype:
Requires acetate at 35 °C; temperature-conditional

For Research Use Only. Not For Clinical Use.