Subategory:

Mutants

Description:

The 70-5Y nuclear mutant was isolated by screening a collection of acetate-requiring mutants for lack of complementation with a known Rubisco rbcL mutant. 70-5Y grows on minimal medium at 25 °C, but dies on minimal medium at 35 °C. When grown with acetate medium at 35 °C, it has normal whole-chain electron transport and chlorophyll levels, but lacks Rubisco holoenzyme. Purified Rubisco has altered catalytic properties. The 70-5Y mutation is not in the Rubisco rbcS genes, and it is not linked to the 68-11AR and 72-7JJ Rubisco nuclear-mutant genes. This strain has been maintained with acetate medium in darkness since its isolation.

Species:

Chlamydomonas