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MUT-4713 rbcL-C449S/C459S mt+ (Moreno)
Catalog No: ALS-03850
Product Details
Subategory:
Mutants
Description:
Directed mutagenesis and chloroplast transformation of rbcL-W66Amber mt+ were used to create C449S (TGT-AGC) and C459S (TGT-TCT) substitutions in the Rubisco large subunit to investigate stress-induced degradation of Rubisco. Although this mutant was originally recovered on minimal medium and could grow photosynthetically, a check of its phenotype revealed that it now requires acetate for growth. Some photosynthesis-competent Rubisco mutants have been previously observed to become acetate-requiring after many years of culture in darkness.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
Phenotype:
Requires acetate
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For Research Use Only. Not For Clinical Use.