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MUT-4727 rbcS1-I58W3 pf2 mt+
Datasheet
Catalog No: ALS-03864
Product Details
Subategory:
Mutants
Description:
Using plasmid p699, rbcL direct mutagenesis, chloroplast transformation of wild-type 2137 mt+ (cloned), selection for spectinomycin resistance in the dark, and screening for a homoplasmic acetate-requiring phenotype to create a K175G substitution (AAA-TCA) in the Rubisco large subunit were performed. The K175S substitution causes a 90% decrease in Rubisco holoenzyme and a 99% decrease in Rubisco carboxylase activity. This mutant was created to investigate the role of active-site Lys-175 in catalysis. It has been maintained with acetate medium in darkness since its creation.
Species:
Chlamydomonas
Locus:
RBCS1, PF2
Chromosome:
2,11
Phenotype:
Requires acetate at 35 °C; temperature-conditional
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For Research Use Only. Not For Clinical Use.