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MUT-4736 rbcS1-Y72A mt-
Datasheet
Catalog No: ALS-03873
Product Details
Subategory:
Mutants
Description:
Directed mutagenesis and nuclear-gene transformation of rbcSΔ-T60-3 mt- were used to create a Y72A substitution in the betaA-betaB loop of the Rubisco small subunit, which causes decreases in Rubisco carboxylase activity and holoenzyme stability. Mutant Y72A has also been studied with respect to enhanced hydrogen production. This mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Species:
Chlamydomonas
Locus:
RBCS1
Chromosome:
2
Phenotype:
Requires acetate at 35 °C; temperature-conditional
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For Research Use Only. Not For Clinical Use.