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MUT-4737 rbcS1-L66F pf2 mt+
Datasheet
Catalog No: ALS-03874
Product Details
Subategory:
Mutants
Description:
Directed mutagenesis and nuclear-gene transformation of rbcSΔ-CAL005.01.13 pf2 mt+ (MUT-4692) were used to create an L66F substitution (CTG-TTC) in the betaA-betaB loop of the Rubisco small subunit, which mimics the biochemical defects of chloroplast Rubisco mutant rbcL-L290F. This mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. It has a cell wall and can be used in genetic crosses. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Species:
Chlamydomonas
Locus:
RBCS1, PF2
Chromosome:
2,11
Phenotype:
Requires acetate at 35 °C; temperature-conditional
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For Research Use Only. Not For Clinical Use.