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MUT-4743 rbcS1-ABEFGP mt-
Datasheet
Catalog No: ALS-03879
Product Details
Subategory:
Mutants
Description:
Using standard methods of genetic engineering and nuclear-gene transformation of rbcSΔ-T60-3 mt- (MUT-4690), the betaA-betaB loop of the Rubisco small subunit was replaced with the small-subunit betaA-betaB loop from Galdieria partita, and residues 128 through 140 were replaced with the small-subunit betaE-betaF loop (residues 105 through 138). These changes cause a decrease in Rubisco CO2/O2 specificity and holoenzyme stability. The mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Species:
Chlamydomonas
Locus:
RBCS1
Chromosome:
2
Phenotype:
Requires acetate at 35 °C; temperature-conditional
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For Research Use Only. Not For Clinical Use.