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MUT-4744 rbcS1-F132Ocher mt- (F132UAA)
Datasheet
Catalog No: ALS-03880
Product Details
Subategory:
Mutants
Description:
Directed mutagenesis and nuclear-gene transformation of rbcSΔ-T60-3 mt- (MUT-4415) were used to change the codon for Phe-132 (TTC) in the Rubisco small subunit to an ocher nonsense codon (TAA), which causes the deletion of nine carboxyl-terminal residues. The mutant Rubisco has decreases in carboxylation catalytic efficiency and thermal stability. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Species:
Chlamydomonas
Locus:
RBCS1
Chromosome:
2
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For Research Use Only. Not For Clinical Use.