Products

Online Inquiry

* Name:

Phone:

* Email:

* Services & Products:

Description:

MUT-4753 rbcS1-E43A mt

Catalog No: ALS-03889

Product Details

Subategory:
Mutants
Description:
Directed mutagenesis and nuclear-gene transformation of rbcSΔ-T60-3 mt- (MUT-4415) were used to create an E43A substitution (GAG-GCC) in the Rubisco small subunit, which causes a decrease in Rubisco CO2/O2 specificity and holoenzyme stability. This mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Species:
Chlamydomonas
Locus:
RBCS1
Chromosome:
2
Phenotype:
Requires acetate at 35 °C; temperature-conditional

For Research Use Only. Not For Clinical Use.