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MUT-4754 rbcS1-E43A pf2 mt+
Datasheet
Catalog No: ALS-03890
Product Details
Subategory:
Mutants
Description:
Using a plasmid that would create an E43A substitution (GAG-GCC) in the Rubisco small subunit, which causes a decrease in Rubisco CO2/O2 specificity and holoenzyme stability, rbcSΔ-CAL005.01.13 pf2 mt+ (MUT-4692) was transformed. In contrast to the E43A mutant described previously, this strain has a cell wall and can be used in genetic crosses. This mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Species:
Chlamydomonas
Locus:
RBCS1, PF2
Chromosome:
2,11
Phenotype:
Requires acetate at 35 °C; temperature-conditional
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For Research Use Only. Not For Clinical Use.