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MUT-4782 rbcL-G171D mt+ (10-6C) (non-pf2 progeny used for genetic analysis)
Datasheet
Catalog No: ALS-03918
Product Details
Subategory:
Mutants
Description:
Following 5-fluorodeoxyuridine treatment and ethyl-methanesulfonate mutagenesis of wild-type 2137 mt+ cells, colonies were screened for light-sensitive, acetate-requiring phenotypes. Mutant 10-6C was found to arise from a G171D substitution (GGT-GAT) in the chloroplast-encoded large subunit of Rubisco, which causes a decrease in Rubisco activities and alters the large-subunit isoelectric point. Selection for photosynthesis-competent revertants yielded only true revertants. This strain was recovered from a cross between 10-6C mt+ (MUT-1815) and pf2 mt-, and has been maintained with acetate medium in darkness.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
Phenotype:
Requires acetate; sensitive to light
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For Research Use Only. Not For Clinical Use.