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MUT-4785 rbcL-W66Amber mt+ (18-7G) (non-pf2 progeny used for genetic analysis)
Datasheet
Catalog No: ALS-03921
Product Details
Subategory:
Mutants
Description:
The 18-7G chloroplast mutant was recovered by screening a collection of acetate-requiring mutants for lack of complementation with a known Rubisco rbcL mutant. It results from an rbcL nonsense mutation that changes the codon for Trp-66 (TGG) to amber (TAG). Selection for photosynthesis-competent revertants yielded heteroplasmic strains that result from chloroplast informational (tRNA) suppression. This strain was recovered from a cross between the original 18-7G mt+ (MUT-2653) and pf2 mt-, and has been used as a host for chloroplast rbcL transformation. It has been maintained with acetate medium in darkness since its isolation.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
Phenotype:
Requires acetate; sensitive to light
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For Research Use Only. Not For Clinical Use.