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MUT-4789 rbcL-R217S/A242V pf2 mt- (R14-A revertant of 28-7J)
Datasheet
Catalog No: ALS-03925
Product Details
Subategory:
Mutants
Description:
R14-A was recovered as a photosynthesis-competent revertant of rbcL-R217S mt+ (28-7J). It results from an intragenic-suppressor mutation that causes an A242V substitution (GCT-GTT) in the Rubisco large subunit, and the double-mutant enzyme has decreases in Rubisco CO2/O2 specificity, holoenzyme stability, and large-subunit isoelectric point. The mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. This strain was recovered from a cross between rbcL-R217S/A242V mt+ and pf2 mt-. It has been maintained with acetate medium in darkness since its isolation.
Species:
Chlamydomonas
Locus:
rbcL, PF2
Chromosome:
Chloroplast,11
Phenotype:
Requires acetate at 35 °C; temperature-conditional
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For Research Use Only. Not For Clinical Use.