Subategory:

Mutants

Description:

Using standard methods of directed mutagenesis and chloroplast-gene transformation of rbcLΔ-25B1 mt+ (MUT-4700), an A242V substitution (GCT-GTT) in the Rubisco large subunit was created. This is the intragenic-suppressor substitution that complements the R217S substitution in photosynthesis-competent revertant R14-A (rbcL-R217S/A242V). Preliminary analysis indicated that the A242V substitution has little or no effect on Rubisco catalysis or holoenzyme stability. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.

Species:

Chlamydomonas

Locus:

rbcL

Chromosome:

Chloroplast