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MUT-4791 rbcL-G54D mt+ (31-4E) (non-pf2 progeny used for genetic analysis)
Datasheet
Catalog No: ALS-03927
Product Details
Subategory:
Mutants
Description:
The 31-4E chloroplast mutant was recovered by screening a collection of acetate-requiring mutants (generated with 5-fluorodeoxyuridine and methyl methanesulfonate) for lack of complementation with a known Rubisco rbcL mutant by standard methods. The mutant was crossed with pf2 mt- to confirm uniparental inheritance, and this mt+ progeny clone was analyzed. Mutant 31-4E results from a G54D substitution (GGT-GAT) in the chloroplast-encoded large subunit of Rubisco, which blocks Rubisco holoenzyme assembly. Selection for photosynthesis-competent revertants yielded true revertants and pseudorevertants. This strain has been maintained with acetate medium in darkness since its isolation.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
Phenotype:
Requires acetate; sensitive to light
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For Research Use Only. Not For Clinical Use.