Subategory:

Mutants

Description:

The 31-4E chloroplast mutant was recovered by screening a collection of acetate-requiring mutants (generated with 5-fluorodeoxyuridine and methyl methanesulfonate) for lack of complementation with a known Rubisco rbcL mutant by standard methods. The mutant was crossed with pf2 mt- to confirm uniparental inheritance, and this pf2 mt- progeny clone was retained for future genetic analysis. Mutant 31-4E results from a G54D substitution (GGT-GAT) in the chloroplast-encoded large subunit of Rubisco, which blocks Rubisco holoenzyme assembly. Selection for photosynthesis-competent revertants yielded true revertants and pseudorevertants. This strain has been maintained with acetate medium in darkness since its isolation.

Species:

Chlamydomonas

Locus:

rbcL, PF2

Chromosome:

Chloroplast,11

Phenotype:

Requires acetate; sensitive to light