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MUT-4797 rbcL-M42V/C53A mt+
Datasheet
Catalog No: ALS-03933
Product Details
Subategory:
Mutants
Description:
Directed mutagenesis and chloroplast transformation of rbcL-W66Amber mt+ were used to create M42V (ATG-GTA) and C53A (TGT-GCT) substitutions in the Rubisco large subunit, which cause a decrease in Rubisco CO2/O2 specificity. This is the original double-mutant strain. It was created to investigate phylogenetic differences near large-subunit residue Gly-54 (see rbcL-G54D). The strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.