Subategory:

Mutants

Description:

This is a stable arginine-requiring strain, easy to score. The arg2 mutation, which was UV-induced, is an allele at the ARG7 locus, encoding argininosuccinate lyase, and is also known as arg7-8. These two mutations are capable of intragenic complementation, and are often used to create diploid strains. Diploids formed between arg2 and the original arg7 mutant are stable and grow at wild type rates in the absence of arginine. Intragenic complementation is also seen between arg2 and arg7-2, arg7-3, arg7-4, arg7-5, arg7-7, and arg7-12. The wild type gene at this locus was cloned, and used to transform arg7 mutants to arginine independence. The arg2 and arg7 mutants require arginine (50 micrograms per mL is recommended) and cannot use ornithine or citrulline. They also grow well on medium containing yeast extract (4 grams per liter).

Species:

Chlamydomonas

Locus:

ARG7

Chromosome:

1

Phenotype:

Requires arginine