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MUT-4803 rbcL-V331A/G344S mt+ (R61-2J revertant of 45-3B) (non-pf2 progeny used for genetic analysis)
Datasheet
Catalog No: ALS-03939
Product Details
Subategory:
Mutants
Description:
R61-2J was recovered as a photosynthesis-competent revertant of rbcL-V331A mt+ (45-3B). It results from an intragenic-suppressor mutation that causes a G344S substitution (GGT-AGT) in the Rubisco large subunit, and the double-mutant enzyme has decreased Rubisco CO2/O2 specificity. The V331A/G344S mutant-enzyme x-ray crystal structure has been solved. This strain was recovered from a cross between rbcL-V331A/G344S mt + and pf2 mt-, and has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function.
Species:
Chlamydomonas
Locus:
rbcL
Chromosome:
Chloroplast
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For Research Use Only. Not For Clinical Use.